The cell section carries the sample parts throughout the column, wherever they interact with the stationary period to different levels. This conversation decides just how long Every single component spends while in the column, causing their separation.

Ion-exchange: Separates charged molecules based on their own interaction with charged purposeful groups on the stationary period.

전자를 '고정상', 후자를 '이동상'이라 부르며 크로마토그래피에서는 분석자는 고정상과 이동상의 조합에 의해 분석물의 분리를 제어할 수 있게 됩니다.따라서 분석물, 고정상, 이동상, 세 가지 특성의 이해가 크로마트그래피에서 매우 중요합니다.

Rotating the interior valve (demonstrated in purple) on the inject place directs the mobile stage through the sample loop and onto the column.

one. The strong-section extraction is essential because it gets rid of constitutions while in the serum that might interfere With all the Investigation. What sorts of interferences are feasible?

The pump is in command of delivering the cellular period at a constant stream price. This makes sure that the cell stage is continually fed into the column.

The detector screens the eluent and generates a sign, which is often in the form of the chromatogram, which is a graphical representation of compound concentration as time passes.

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The three pink circles are binary cellular phases made by combining equivalent volumes on the pure cellular phases. The ternary mobile section demonstrated through the purple circle incorporates all a few with the pure mobile phases.

The column may be the separation chamber exactly where the magic of HPLC transpires. It residences the stationary stage, a packed mattress of microscopic particles.

The pressurized liquid is often a mixture of solvents for example drinking water, acetonitrile and/or methanol which is called the cellular phase.

검토 중에서 컬럼이나 이동상 등 여러 조건의 조합은 분석 가능성의 큰 영향을 미칩니다.)

The concentration of caffeine in beverages is set by a reversed-section HPLC separation employing a mobile period of 20% acetonitrile and 80% read more h2o, and using a nonpolar C8 column. Effects for your series of ten-μL injections of caffeine specifications are in the subsequent desk.